PORTO-
FREI

J S, J: Bt isolates from India: Bioefficacy and molecular ch

Insect pathology and biological control

von J S, Jacob / Mathew M P, Dr. / Girija D, Dr.   (Autor)

Bacillus thuringiensis Berliner is a gram-positive, spore forming bacterium that produces proteinaceous crystal containing insecticidal toxins. The pathogenicity of 20 B. thuringiensis isolates, collected from the Western Ghats of Kerala, were evaluated. The tobacco caterpillar, Spodoptera litura Fb. was used as the test insect. Three isolates were found to be toxic to S. litura during preliminary screening. A standardized bioassay carried out with five different concentrations of all the selected isolates and the reference standard HD-1, indicated the equal effectiveness of the native isolates with the standard strain. The lowest LC50 was obtained in KAU-51 and highest in KAU-11. The lowest LT50 was recorded in HD-1.The molecular characterization of the selected isolates was performed with RAPD-PCR technique. Ten primers, which produced more than five bands were selected for RAPD analysis. The RAPD data was used to generate a similarity matrix using the NTSyS programme. The results showed that high variability exists between the selected isolates.

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Produktbeschreibung

Bacillus thuringiensis Berliner is a gram-positive, spore forming bacterium that produces proteinaceous crystal containing insecticidal toxins. The pathogenicity of 20 B. thuringiensis isolates, collected from the Western Ghats of Kerala, were evaluated. The tobacco caterpillar, Spodoptera litura Fb. was used as the test insect. Three isolates were found to be toxic to S. litura during preliminary screening. A standardized bioassay carried out with five different concentrations of all the selected isolates and the reference standard HD-1, indicated the equal effectiveness of the native isolates with the standard strain. The lowest LC50 was obtained in KAU-51 and highest in KAU-11. The lowest LT50 was recorded in HD-1.The molecular characterization of the selected isolates was performed with RAPD-PCR technique. Ten primers, which produced more than five bands were selected for RAPD analysis. The RAPD data was used to generate a similarity matrix using the NTSyS programme. The results showed that high variability exists between the selected isolates. 

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LAP Lambert Acad. Publ.

Produktdetails

Medium: Buch
Format: Kartoniert
Seiten: 136
Sprache: Englisch
Erschienen: Februar 2011
Sonstiges: .
Maße: 220 x 150 mm
Gewicht: 219 g
ISBN-10: 3844306226
ISBN-13: 9783844306224

Bestell-Nr.: 10362401 
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KNOABBVERMERK: 2011. 136 S.
KNOSONSTTEXT: .
Einband: Kartoniert
Sprache: Englisch
Beilage(n): Paperback

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