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Capillary Electrophoresis

Methods and Potentials

von Beck, Wolfgang / Engelhardt, Heinz / Schmitt, Thomas   (Autor)

Capillary electrophoresis (CE), also designated by the acronym HPCE for "High Per formance Capillary Electrophoresis" is a rapidly growing analytical separation method. It unites the separation technique of classical electrophoresis on plates with the instrumental methods of chromatography with respect to direct detection of the solutes separated in the capillary and their ready identification and quantification. The initial problems of inadequate reproducibility in quantitative analysis, due to the necessity of handling extremely small volumes, have largely been solved in the sec ond generation commercial instruments. Hence, a rapid and reliable separation sys tem is available for ionic compounds from the smallest cation (the lithium ion) up to poly anions with molecular weights ranging in the millions (such as DNA molecules). The methods of gel electrophoresis and isoelectric focusing can be readily extended to separation techniques carried out in a capillary. For nonionic compounds an addi tional separation method is available in the form of micellar electrokinetic chroma tography (MEKC). This involves a true chromatographic separation process because the distribution of the analytes between the buffer and the micelles is superimposed on the electrophoretic migration, which contributes substantially to the selectivity.

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Produktbeschreibung

Capillary electrophoresis (CE), also designated by the acronym HPCE for "High Per formance Capillary Electrophoresis" is a rapidly growing analytical separation method. It unites the separation technique of classical electrophoresis on plates with the instrumental methods of chromatography with respect to direct detection of the solutes separated in the capillary and their ready identification and quantification. The initial problems of inadequate reproducibility in quantitative analysis, due to the necessity of handling extremely small volumes, have largely been solved in the sec ond generation commercial instruments. Hence, a rapid and reliable separation sys tem is available for ionic compounds from the smallest cation (the lithium ion) up to poly anions with molecular weights ranging in the millions (such as DNA molecules). The methods of gel electrophoresis and isoelectric focusing can be readily extended to separation techniques carried out in a capillary. For nonionic compounds an addi tional separation method is available in the form of micellar electrokinetic chroma tography (MEKC). This involves a true chromatographic separation process because the distribution of the analytes between the buffer and the micelles is superimposed on the electrophoretic migration, which contributes substantially to the selectivity. 

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Produktdetails

Medium: Buch
Format: Kartoniert
Seiten: 228
Sprache: Englisch
Erschienen: Juni 2012
Auflage: 1996
Maße: 245 x 172 mm
Gewicht: 410 g
ISBN-10: 3642858562
ISBN-13: 9783642858567

Bestell-Nr.: 15231441 
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KNOABBVERMERK: 1996. 2012. x, 215 S. 19 SW-Abb. 244 mm
KNOMITARBEITER: Übersetzung: Gutnikov, G.
Einband: Kartoniert
Auflage: 1996
Sprache: Englisch
Beilage(n): Paperback

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